The effects of BuzzClear, known in experimental studies as Alcohol Degredation Product (ADP), was investigated by an independent laboratory in a series of in vitro and in vivo animal experiments. This research was published in the journal Foods in 2024.

In the first experiment, liver cells growing in a cell culture, specifically the HepG2 cell line, were challenged with 5% alcohol for 24 hours. In one set of liver cells, the cells were pre-treated with ADP. The other set of liver cells did not get pre-treated with ADP. The liver cells pretreated with ADP showed 90% viability versus 64% viability of those cells that did not get ADP. This shows that the enzymes in BuzzClear were able to keep significantly more liver cells alive when exposed to alcohol.  In summary, we found that BuzzClear was non-toxic to liver cells and may protect against alcohol induced liver toxicity.

In an animal experiment using zebrafish in an aquarium, ADP’s effect on swimming behavior when exposed to alcohol was studied. Zebrafish given 2% alcohol in their tanks showed reduced swimming distance, velocity, and swimming activity time. In a tank where ADP was introduced into the tank, the reduced zebrafish activities were significantly alleviated.  DP may help metabolize the alcohol in the aquarium allowing the zebrafish to regain normal swimming activity. 

The last experiment  was an animal study looking at alcohol’s effect on the liver of laboratory rats and whether or not ADP can mitigate its effects. One group of animals were given ADP 30 minutes before given 3g/kg (30%) alcohol.  nother group of animals did not receive ADP prior to being given the same amount of alcohol. A third control group of animals did not receive ADP or alcohol for comparison

Several effects were observed.  

First, the blood alcohol concentration of the ADP pre-treated group was significantly lower than the non-preated group 1 hour after alcohol ingestion.  

Second, a histopathological analysis showed that there was significantly more damage to the livers of animals exposed to alcohol as compared to those animals pre-treated with ADP. Animals pre-treated with ADP looked similar to control animals that never ingested alcohol.  

Third, the enzymatic activity of ADP was found to work in the small intestine or the gut before alcohol can enter the bloodstream. Having both alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) work in the gut is important so that the toxic acetaldehyde is metabolized and excreted before it can enter the body.

And lastly, ADP inhibits the over-activation of an acute-phase-reactant protein known as heme-oxygenase-1 (HO-1) and thereby may reduce oxidative stress, inflammation and potential scarring of the liver which can occur after drinking alcohol. Acute-phase-reactant proteins are present when the body is experiencing significant stress and in the liver, HO-1 expression increases when the liver is being harmed.

In summary, BuzzClear, a dual-enzyme supplement (Alcohol Degradation Product or ADP), protects liver cells, mitigates ethanol-induced behavioral impairment, and reduces blood alcohol concentrations in animal models by promoting first-pass metabolism in the gut. This research shows that BuzzClear may significantly reduce liver damage and oxidative stress by breaking down alcohol and acetaldehyde before they enter the bloodstream. 

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